Activated carbon: ndi mtundu wa non-polar adsorbent ntchito kwambiri. Nthawi zambiri, iyenera kutsukidwa ndi dilute hydrochloric acid, kenako Mowa, kenako kutsukidwa ndi madzi. Mukaumitsa pa 80 ℃, imatha kugwiritsidwa ntchito ngati chromatography. Granular activated carbon ndiye chisankho chabwino kwambiri pa column chromatography. Ngati ndi ufa wabwino wa carbon activated, m'pofunika kuwonjezera mlingo woyenerera wa diatomite ngati wothandizira fyuluta, kuti mupewe kuthamanga kwapang'onopang'ono.
Activated carbon ndi non-polar adsorbent. Kutsatsa kwake kumatsutsana ndi silika gel ndi alumina. Ili ndi mgwirizano wamphamvu wazinthu zomwe sizili polar. Ili ndi mphamvu yamphamvu ya adsorption mu njira yamadzimadzi komanso yofooka mu zosungunulira za organic. Choncho, mphamvu elution ya madzi ndi chofooka ndi zosungunulira organic ndi wamphamvu. Pamene adsorbed mankhwala eluted kuchokera adamulowetsa mpweya, ndi polarity wa zosungunulira amachepetsa, ndi adsorption mphamvu ya solute pa adamulowetsa mpweya amachepetsa, ndi elution mphamvu ya eluent kumatheka. Zigawo zosungunuka m'madzi, monga ma amino acid, shuga ndi glycosides, zidapatulidwa.